Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MDA-MB-231
cell line
MDA-MB-231
cell type
16h MDA-MB-231 mammospheres
chip antibody
EZH2 (Active Motif, Catalog# 39875)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Following ChIP protocol, DNA was recovered using phenol-chloroform extraction protocol. Libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® following the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
101160351
Reads aligned (%)
95.8
Duplicates removed (%)
52.5
Number of peaks
5844 (qval < 1E-05)

hg19

Number of total reads
101160351
Reads aligned (%)
95.5
Duplicates removed (%)
52.7
Number of peaks
5969 (qval < 1E-05)

Base call quality data from DBCLS SRA