Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

embryonic cells

cell line

S2

chip antibody

none

genotype/variation

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates from embryonic cells or 3rd instar brains were clarified from sonicated nuclei and lamin-DNA complexes were isolated with specific antibody Libraries were prepared according to Illumina's instructions for HiSeq 2000 for KC or S2 cells and NextSeq 500/550 High Output Kit when brains were analyzed.

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

19970393

Reads aligned (%)

97.8

Duplicates removed (%)

11.3

Number of peaks

4323 (qval < 1E-05)

dm3

Number of total reads

19970393

Reads aligned (%)

98.0

Duplicates removed (%)

9.4

Number of peaks

3934 (qval < 1E-05)