Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cell line

Cell type

Kc167

Source

e/se

Developmental Stage

dorsal closure stage

Sample

source_name

embryonic cells

cell line

KC

chip antibody

none

genotype/variation

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates from embryonic cells or 3rd instar brains were clarified from sonicated nuclei and lamin-DNA complexes were isolated with specific antibody Libraries were prepared according to Illumina's instructions for HiSeq 2000 for KC or S2 cells and NextSeq 500/550 High Output Kit when brains were analyzed.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

32104277

Reads aligned (%)

97.8

Duplicates removed (%)

11.9

Number of peaks

3837 (qval < 1E-05)

dm3

Number of total reads

32104277

Reads aligned (%)

97.9

Duplicates removed (%)

9.3

Number of peaks

0 (qval < 1E-05)