Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF398

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA

Attributes by original data submitter

Sample

source_name
H9 hESCs
cell type
H9
antibody
ZNF398

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After decrosslinking, DNA was purified using QIAQuick PCR Purification Kit (Qiagen) according to the manufacturer's instructions. ChIP-seq library was prepared with approximately 5 ng of immunoprecipitated DNA were used as input for the NEBNext® ChIP-Seq Library Prep kit, following manufacturer's instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
29300460
Reads aligned (%)
8.5
Duplicates removed (%)
90.0
Number of peaks
657 (qval < 1E-05)

hg38

Number of total reads
29300460
Reads aligned (%)
9.6
Duplicates removed (%)
88.8
Number of peaks
679 (qval < 1E-05)

Base call quality data from DBCLS SRA