Antigen

Antigen Class

TFs and others

Antigen

ZNF398

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

H9 hESCs

cell type

H9

antibody

ZNF398

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After decrosslinking, DNA was purified using QIAQuick PCR Purification Kit (Qiagen) according to the manufacturer's instructions. ChIP-seq library was prepared with approximately 5 ng of immunoprecipitated DNA were used as input for the NEBNext® ChIP-Seq Library Prep kit, following manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

29300460

Reads aligned (%)

85.6

Duplicates removed (%)

88.8

Number of peaks

679 (qval < 1E-05)

hg19

Number of total reads

29300460

Reads aligned (%)

85.1

Duplicates removed (%)

90.0

Number of peaks

657 (qval < 1E-05)