GSM1469984: RARa ZF BiotinChIPseq Dox RA E2 Exp2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Biotin
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF7 Tet-On Inducible stable line
cell line
MCF7
cell description
beast cancer cell line
chip antibody or biotin-streptavidin pulldown of blrp-tagged proteins
Biotin-ChIP using Streptavidin Magnetic beads (Solulink)
treatment
24hrs treatment with 2μg/ml doxycycline, 1hr treatment with 1μM RA and 100nM E2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody or by streptavidin magnet beads pull-down. The extracted ChIP DNA was ligated to specific adaptors of Truseq system from Illumina, according to the manufacturer’s instructions.