Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Neural
Cell type
Cerebellum
MeSH Description
The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.

Attributes by original data submitter

Sample

source_name
Cerebellum
strain
C57BL/6
tissue
Cerebellum
treatment
EOD
age
3M before expt, 6M after expt
chip antibody
Anti-histone H3 (tri methyl K9) antibody- ChIP grade ab8898, abcam

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cerebellum was harvested, immediately flash frozen and stored at -80°C. ChIP & RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
15266270
Reads aligned (%)
92.8
Duplicates removed (%)
27.1
Number of peaks
714 (qval < 1E-05)

mm9

Number of total reads
15266270
Reads aligned (%)
92.7
Duplicates removed (%)
26.9
Number of peaks
680 (qval < 1E-05)

Base call quality data from DBCLS SRA