Antigen

Antigen Class

TFs and others

Antigen

Hells

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

B6-Prdm9CAST-KI_testis_ChIP-seq

strain

B6-Prdm9CAST-KI

age

12-14 days post-partum

tissue/cell type

testis

chromatin fragmentation

Sonication

chip antibody

HELLS (Millipore/Sigma, ABD41 lot# 3069868)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

KAPA hyper kit See Samples section

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

60724234

Reads aligned (%)

98.1

Duplicates removed (%)

24.7

Number of peaks

472 (qval < 1E-05)

mm9

Number of total reads

60724234

Reads aligned (%)

97.9

Duplicates removed (%)

24.7

Number of peaks

522 (qval < 1E-05)