Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

B6-Prdm9CAST-KI_testis_ChIP-seq

strain

B6-Prdm9CAST-KI

age

12-14 days post-partum

tissue/cell type

testis

chromatin fragmentation

Sonication

chip antibody

IgG (Millipore/Sigma #12-371, lot: 2880788)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

KAPA hyper kit See Samples section

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

43232463

Reads aligned (%)

80.8

Duplicates removed (%)

37.3

Number of peaks

462 (qval < 1E-05)

mm9

Number of total reads

43232463

Reads aligned (%)

80.6

Duplicates removed (%)

37.3

Number of peaks

444 (qval < 1E-05)