Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Gonad
Cell type
Male germ cells
NA
NA

Attributes by original data submitter

Sample

source_name
C57BL/6J_male germ cells_ChIP-seq
strain
C57BL/6J
age
12-14 days post-partum
tissue/cell type
male germ cells
chromatin fragmentation
MNase
chip antibody
H3K27ac (abcam #ab4729; lot:GR211893-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Bioo Scientific's NEXTflex ChIP-Seq Kit (protocol version V11,11) See Samples section

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
53294241
Reads aligned (%)
91.8
Duplicates removed (%)
7.5
Number of peaks
990 (qval < 1E-05)

mm9

Number of total reads
53294241
Reads aligned (%)
91.7
Duplicates removed (%)
7.5
Number of peaks
1016 (qval < 1E-05)

Base call quality data from DBCLS SRA