Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZNF486

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
embryonic stem cells, ZNF486 ChIP
transchromosomic
no
plasmid
none
chip_or_input
ZNF486 ChIP
chip antibody
ZNF486 (abcam ab104878, lot#GR52278-1)
nickname
KZNF (hESC) r1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
O'Geen H, Echipare L, Farnham PJ. Using ChIP-seq technology to generatehigh-resolution profiles of histone modifications. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20. PubMed PMID: 21913086. TrueSeq DNA library prep, low input protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
84026289
Reads aligned (%)
82.9
Duplicates removed (%)
51.0
Number of peaks
3620 (qval < 1E-05)

hg38

Number of total reads
84026289
Reads aligned (%)
85.3
Duplicates removed (%)
50.2
Number of peaks
5286 (qval < 1E-05)

Base call quality data from DBCLS SRA