Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Embryo

Cell type

Parasegment 7

NA

NA

Sample

source_name

Pol2_PS7

tissue

embryo

age

5-13h

tissue subtype

parasegment 7

chip antibody

anti-RNA Pol II phospho-S5

chip antibody vendor

Abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nuclei were sonicated and ChIP performed using the appropriate antibody. Libraries were prepared as described in Bowman et al., 2013.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

53639490

Reads aligned (%)

73.8

Duplicates removed (%)

89.5

Number of peaks

4529 (qval < 1E-05)

dm3

Number of total reads

53639490

Reads aligned (%)

74.0

Duplicates removed (%)

88.8

Number of peaks

6342 (qval < 1E-05)