GSM1466896: Input DNA me-2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
MCF-7 Breast cancer
cell line
MCF-7
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq libraries were prepared from 10ng fragmented DNA using the NEBNext ChIP-Seq Library Prep Master Mix Kit (NEB-E6240S). Accordingly, after end-repair and A-tailing sequencing, adaptors were ligated and size selection of adaptor-ligated DNA using AMPure XP Beads (Beckman Coulter) performed. Libraries were enriched with 12 PCR cycles and sequenced according to manufacturer’s protocol on an Illumina HiSeq2000 instrument