SRX6708987
GSM4030115: Splenic aCD40 IL4 Rad21 2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rad21

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
Splenic mature B cells
cell type
AID-deficient Splenic mature B cells
passages
0 (primary mature splenic B cells)
strain
C57BL/6
chip antibody
Rad21 (Abcam, ab992)
treatment
with aCD40/IL4 stimulation

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
NextSeq 550

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
51671223
Reads aligned (%)
95.7
Duplicates removed (%)
64.0
Number of peaks
31081 (qval < 1E-05)

mm9

Number of total reads
51671223
Reads aligned (%)
95.7
Duplicates removed (%)
64.1
Number of peaks
31039 (qval < 1E-05)

Base call quality data from DBCLS SRA