SRX6708980
GSM4030108: CH12F3NCdel aCD40 IL4 TGFb Rad21 4; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rad21

Cell type

Cell type Class
Blood
Cell type
CH12F3
NA
NA

Attributes by original data submitter

Sample

source_name
CH12F3 murine lymphoma cells
cell type
AID-deficient Splenic mature B cells
passages
8--17
strain
C57BL/6
chip antibody
Rad21 (Abcam, ab992)
treatment
with aCD40/IL4/TGFb stimulation

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
NextSeq 550

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
45620719
Reads aligned (%)
98.2
Duplicates removed (%)
33.1
Number of peaks
23212 (qval < 1E-05)

mm9

Number of total reads
45620719
Reads aligned (%)
98.1
Duplicates removed (%)
33.4
Number of peaks
23241 (qval < 1E-05)

Base call quality data from DBCLS SRA