Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
CH12F3
NA
NA

Attributes by original data submitter

Sample

source_name
CH12F3 murine lymphoma cells
cell type
AID-deficient Splenic mature B cells
passages
8--10
strain
C57BL/6
chip antibody
none
treatment
with aCD40/IL4/TGFb stimulation

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the TruSeq ChIP Library Preparation Kit (IP-202-1012).

Sequencing Platform

instrument_model
NextSeq 550

mm10

Number of total reads
42126554
Reads aligned (%)
98.7
Duplicates removed (%)
25.3
Number of peaks
199 (qval < 1E-05)

mm9

Number of total reads
42126554
Reads aligned (%)
98.6
Duplicates removed (%)
25.5
Number of peaks
194 (qval < 1E-05)

Base call quality data from DBCLS SRA