GSM4025401: Input DNA control shRNA; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Undifferentiated mouse embryonic stem cell line EL 16.7
strain/background
F2: 129S1/CastEiJx 129S1
genotype/variation
Wild type
cell type
Undifferentiated embryonic stem cells
Sex
female
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DamID was performed as described in Vogel at al. Nat Protoc. 2007; 2(6):1467-78. ChIP-Seq was performed as described in Kadota and Nagata Nucleic Acids Res. 2014; 42(12):7642-53. For DamID-Seq, purified methyl PCR products were digested with Dpn II to remove adapter sequences from the fragment ends and 30 ng of PCR products were treated as DNA templates to prepare paired-end Solexa libraries as previously described Yildirim et al. Nat Struct Mol Bio 2011; 19(1):56-61.