Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC HUES9

NA

NA

Sample

source_name

HUES9 hESC 1.8 subline

cell line

HUES9 hESC subline

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 2mM disuccinimidyl glutarate (DSG) for 45 mins and 1% formaldehyde for 10mins, and followed by glycine for 5 mins. Chromatin DNA was sheared to 200–500 bp average in size by sonication and chromatin was immunoprecipitated with antibodies. Protein A/G beads Sigma were added and incubated overnight at 4°C. After washing and elution, the protein–DNA complex was reverse-crosslinked by heating at 65°C, and immunoprecipitated DNA was purified by using QIAquick Spin column. ChIP-seq libraries were prepared using the KAPA Library Preparation Kit (KK8201)

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

17439620

Reads aligned (%)

85.8

Duplicates removed (%)

1.5

Number of peaks

14566 (qval < 1E-05)

hg19

Number of total reads

17439620

Reads aligned (%)

85.0

Duplicates removed (%)

2.7

Number of peaks

14570 (qval < 1E-05)