Embryonic day 14.5 fetal liver hematopoietic stem cells
strain
B6129SF1
tissue
Fetal liver
age
Embryonic day 14.5
chip antibody
H3K27ac (ab4729, Abcam)
cell type
fetal liver hematopoietic stem cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
5 stages of hematopoietic stem and progenitor cells were sorted from mouse embryos or adult bone marrow. ChIP reactions were performed using 20,000 FACS-sorted cells. Iped and Input DNA were purified with MinElute PCR Purification Kit. Libraries were constructed using Rubicom ThruPLEX DNA-Seq Kit. Libraries were size-selected at 100-700 bp by AMPure XP beads. Libraries were quantified with KAPA qPCR and bioanalyzer prior to single-end sequencing.