GSM4018840: DKO ChIPseq HP1alpha rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CBX5
Cell type
Cell type Class
Digestive tract
Cell type
DKO
NA
NA
Attributes by original data submitter
Sample
source_name
DKO_ChIPseq_HP1alpha
cell line
DKO
cell type
colon cancer cell line
cell line source gender
male
library strategy
ChIP-seq
chip antibody
anti-HP1alpha (CST:2616)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown to ~75% confluency, harvested with trypsin, washed in 1X PBS, and frozen/stored at -80°C. Thawed cells were fixed in 1% formaldehyde and quenched in 0.125M Glycine, then washed twice in 1X PBS. Fixed cells were then resuspended in 500ul lysis buffer (50mM Tris-HCl pH 8.0, 10mM NaCl, 0.2% NP40, 1X PITC) for 30 min on ice with periodic resuspension. Libraries were prepared using NEB UltraII DNA library kit