GSM4018756: LNCAP DMSO H3K79ME2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K79me2
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
LNCaP
cell line
LNCaP
tissue
Prostate cell line
chip antibody
H3K79me2 (Abcam; ab177184)
treatment
DMSO
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with formaldehyde and lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Barcoded sequencing libraries were generated using KAPA Library Preparation Kits (Kapa Biosystems) according to the manufacturer's protocol. Libraries were quantified using a qPCR-based quantification (Kapa Biosystems).