SRX6665829
GSM4012668: WT H3Ac ChIPSeq Rep2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3ac

Cell type

Cell type Class
Blood
Cell type
Myeloid-derived suppressor cells
NA
NA

Attributes by original data submitter

Sample

source_name
S. aureus infected orthopedic implant-associated tissue
cell type
MDSCs
strain
C57BL/6
age
10-12 weeks
infected with
WT S. aureus
chip antibody
Histone H3ac (pan-acetyl, pAb) antibody (Active motif Cat no. 61537)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Soft tissues surrounding S. aureus-infected implants were removed, homogenized and stained for FACS. Live MDSCs from WT and triple mutant-infected animals were sorted and chromatin isolated from each sample.

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
34824874
Reads aligned (%)
85.8
Duplicates removed (%)
34.7
Number of peaks
402 (qval < 1E-05)

mm9

Number of total reads
34824874
Reads aligned (%)
85.6
Duplicates removed (%)
34.8
Number of peaks
386 (qval < 1E-05)

Base call quality data from DBCLS SRA