Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Monocytes-CD14+

Tissue

monocytes

Lineage

mesoderm

Description

Monocytes-CD14+ are CD14-positive cells from human leukapheresis production, from donor RO 01746 (draw 1 ID is RO 01746, draw 2 ID is RO 01826), newly promoted to tier 2: not in 2011 analysis

Sample

source_name

monocytes from healthy human donnor

subject status

Healthy control

tissue

Peripheral Blood

cell type

monocyte CD14+

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

20000 to 50000 sorted monocytes were washed with cold PBS and resuspended in cold lysis buffer (10 mM Tris·Cl, pH 7.4, 10 mM NaCl,3 mM MgCl2, 0.1% (v/v) Igepal CA-630). Nuclei were subject to transposition reaction in 1x TD Buffer containing 2.5 μl transposase Nextera enzyme and DNA was purified. Purified DNA was amplified by PCR with 10-12 cycles. Amplified libraries were purified and quality controlled.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

39368444

Reads aligned (%)

63.4

Duplicates removed (%)

38.8

Number of peaks

33162 (qval < 1E-05)

hg19

Number of total reads

39368444

Reads aligned (%)

63.1

Duplicates removed (%)

39.0

Number of peaks

32972 (qval < 1E-05)