Antigen

Antigen Class

Histone

Antigen

TH2BS11ph

Cell type

Cell type Class

Gonad

Cell type

Spermatocytes

MeSH Description

Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.

Sample

source_name

Testicular cells

strain

C57BL6

cell type

Pachytene spermatocytes

age

post natal day 20

chip antibody

TH2BS11ph antibody

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Mononucleosomes were isolated from mouse testicular cells (P12 and P20). Immunoprecipitation were carried out with the TH2BS11ph antibody. After washing of beads, DNA were extracted using phenol-choloroform method. Libraries were prepared using Nebnext ultra DNA library preparation kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

47175174

Reads aligned (%)

83.1

Duplicates removed (%)

33.4

Number of peaks

494 (qval < 1E-05)

mm9

Number of total reads

47175174

Reads aligned (%)

82.8

Duplicates removed (%)

33.4

Number of peaks

482 (qval < 1E-05)