Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

Gastric cancer cell line

NA

NA

Sample

source_name

Gastric cancer cell line

cell type

Gastric cancer cell line

chip antibody

H3K27ac_ChIP (Millipore, 05-1334, lot 2768425)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA complexes were isolated. ChIP-seq libraries were prepared using KAPA Hyper Prep Kit (KAPA Biosystems) following the manufacturer's protocol.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

26052673

Reads aligned (%)

97.7

Duplicates removed (%)

5.9

Number of peaks

26092 (qval < 1E-05)

hg19

Number of total reads

26052673

Reads aligned (%)

97.3

Duplicates removed (%)

6.7

Number of peaks

26804 (qval < 1E-05)