Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Unclassified

Cell type

Unclassified

NA

NA

Sample

source_name

Primograft_H3K79me3_Input

tissue

L826

cell type

Presentation ALL patient sample engrafted in mice and harvested from spleen

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (EZH2). Cells were sonicated to give fragments of 100-300bp followed by IP. DNA libraries were made using the NEBnext ultra DNA library preparation kit for Illumina (Cat no. E7370).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

48571409

Reads aligned (%)

87.1

Duplicates removed (%)

1.5

Number of peaks

1575 (qval < 1E-05)

hg19

Number of total reads

48571409

Reads aligned (%)

86.1

Duplicates removed (%)

2.2

Number of peaks

1105 (qval < 1E-05)