Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Neural

Cell type

Prefrontal Cortex

MeSH Description

The rostral part of the frontal lobe, bounded by the inferior precentral fissure in humans, which receives projection fibers from the MEDIODORSAL NUCLEUS OF THE THALAMUS. The prefrontal cortex receives afferent fibers from numerous structures of the DIENCEPHALON; MESENCEPHALON; and LIMBIC SYSTEM as well as cortical afferents of visual, auditory, and somatic origin.

Sample

source_name

WIN+cocaine-treated

tissue

PFC

strain

Sprague-Dawley

Sex

male

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Total RNA was harvested using Trizol reagent. KAPA Stranded RNA-Seq Library Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. ATAC-seq: Nuclei from flash frozen PFC tissue were extracted and 100,000 nuclei were tagmented. DNA were prepared using Nextera library prep kit. RNA and DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

NextSeq 500

rn6

Number of total reads

63472740

Reads aligned (%)

83.9

Duplicates removed (%)

25.4

Number of peaks

99992 (qval < 1E-05)