Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

CD8+ T cells

NA

NA

Sample

source_name

T CD8+ cells

cell type

T CD8+ cells

passages

In vivo FACS purified cells

strain

C57BL/6

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

21738120

Reads aligned (%)

98.0

Duplicates removed (%)

63.5

Number of peaks

6257 (qval < 1E-05)

mm9

Number of total reads

21738120

Reads aligned (%)

97.8

Duplicates removed (%)

63.7

Number of peaks

6195 (qval < 1E-05)