Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

Short Term Hematopoietic Stem Cell (ST-HSC)

cell type

Short Term Hematopoietic Stem Cell (ST-HSC)

passages

In vivo FACS purified cells

strain

C57BL/6

chip antibody

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

15452492

Reads aligned (%)

98.2

Duplicates removed (%)

57.2

Number of peaks

14772 (qval < 1E-05)

mm9

Number of total reads

15452492

Reads aligned (%)

98.0

Duplicates removed (%)

57.3

Number of peaks

14768 (qval < 1E-05)