Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Monocytes

MeSH Description

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Sample

source_name

Monocytes

cell type

Monocytes

passages

In vivo FACS purified cells

strain

C57BL/6

chip antibody

H3K4me2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

31670529

Reads aligned (%)

98.5

Duplicates removed (%)

69.3

Number of peaks

16572 (qval < 1E-05)

mm9

Number of total reads

31670529

Reads aligned (%)

98.3

Duplicates removed (%)

69.4

Number of peaks

16481 (qval < 1E-05)