Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Blood

Cell type

Granulocytes

MeSH Description

Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.

Sample

source_name

Granulocytes

cell type

Granulocytes

passages

In vivo FACS purified cells

strain

C57BL/6

chip antibody

H3K4me2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

24307399

Reads aligned (%)

98.4

Duplicates removed (%)

65.6

Number of peaks

20900 (qval < 1E-05)

mm9

Number of total reads

24307399

Reads aligned (%)

98.2

Duplicates removed (%)

65.7

Number of peaks

20937 (qval < 1E-05)