Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me2

Cell type

Cell type Class
Blood
Cell type
Granulocytic-monocytic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
Granulocyte Monocyte Progenitor (GMP)
cell type
Granulocyte Monocyte Progenitor (GMP)
passages
In vivo FACS purified cells
strain
C57BL/6
chip antibody
H3K4me2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
46292025
Reads aligned (%)
98.4
Duplicates removed (%)
75.3
Number of peaks
26795 (qval < 1E-05)

mm9

Number of total reads
46292025
Reads aligned (%)
98.3
Duplicates removed (%)
75.4
Number of peaks
26840 (qval < 1E-05)

Base call quality data from DBCLS SRA