Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Multipotent progenitors

NA

NA

Sample

source_name

Multipotent Progenitor (MPP)

cell type

Multipotent Progenitor (MPP)

passages

In vivo FACS purified cells

strain

C57BL/6

chip antibody

H3K27Ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. A new protocol (iChIP) was designed to perform iCHIP from small cell numbers

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

8739264

Reads aligned (%)

98.3

Duplicates removed (%)

52.3

Number of peaks

4478 (qval < 1E-05)

mm9

Number of total reads

8739264

Reads aligned (%)

98.2

Duplicates removed (%)

52.4

Number of peaks

4491 (qval < 1E-05)