Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
MEFs cells knockout MED23
cell type
mouse embryonic fibroblast
genotype/variation
MED23 knockout

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysates were sonicated and immunoprecipitated DNA-protein complex using antibody. ChIP DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
38530359
Reads aligned (%)
98.6
Duplicates removed (%)
12.3
Number of peaks
465 (qval < 1E-05)

mm9

Number of total reads
38530359
Reads aligned (%)
98.5
Duplicates removed (%)
12.4
Number of peaks
471 (qval < 1E-05)

Base call quality data from DBCLS SRA