Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

MEFs cells wildtype

cell type

mouse embryonic fibroblast

genotype/variation

wildtype

chip antibody

Pol II

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysates were sonicated and immunoprecipitated DNA-protein complex using antibody. ChIP DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

38376118

Reads aligned (%)

96.1

Duplicates removed (%)

19.6

Number of peaks

10960 (qval < 1E-05)

mm9

Number of total reads

38376118

Reads aligned (%)

95.9

Duplicates removed (%)

19.7

Number of peaks

10993 (qval < 1E-05)