Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
C2 iMEFs Ezh1 pre-rescue condition
cell type
iMEFs
antibody
H3K27me3 ChIP (clone 7b11)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted by phenol-chloroform and precipitated. Experiment 1 : Sequencing was performed on SOLiD 5500 single reads 75bp. Experiment 2 : Sequencing was performed on Hi-Seq 2500 Illumina single reads 100bp.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
57900625
Reads aligned (%)
99.3
Duplicates removed (%)
10.8
Number of peaks
180 (qval < 1E-05)

mm9

Number of total reads
57900625
Reads aligned (%)
99.2
Duplicates removed (%)
10.8
Number of peaks
149 (qval < 1E-05)

Base call quality data from DBCLS SRA