GSM3964232: input DNA (ChIP); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
pooled
cell type
pooled
chip antibody
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were processed by Active Motif via a standard ChIPseq protocol to enrich for RNAPII bound regions of DNA. Replicate RNA Pol II ChIP reactions were performed using 25 μg of primary MEF, 6F, and DDRR chromatin and 4 μg of Abflex RNAPII antibody (Active Motif, cat # 91151). Libraries were generated via standard Illumina protocols (using NextSeq 500) and sequenced to generate 30M reads per sample.