Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Embryo

Cell type

0-16h embryos

NA

NA

Sample

source_name

0-16h embryos

antibody

-

tissue

0-16h embryos

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody, as described in Orlando, et al., Embo J (1998) Sequencing libraries were prepared with the KAPA Library Preparation Kit Illumina series (KK8201) according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

22022780

Reads aligned (%)

95.7

Duplicates removed (%)

11.8

Number of peaks

2088 (qval < 1E-05)

dm3

Number of total reads

22022780

Reads aligned (%)

96.3

Duplicates removed (%)

9.7

Number of peaks

0 (qval < 1E-05)