Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Embryo

Cell type

0-16h embryos

NA

NA

Sample

source_name

0-16h embryos

antibody

anti-H3K27me3, CS200603 (Milipore)

tissue

0-16h embryos

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody, as described in Orlando, et al., Embo J (1998) Sequencing libraries were prepared with the KAPA Library Preparation Kit Illumina series (KK8201) according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

13124734

Reads aligned (%)

92.7

Duplicates removed (%)

20.3

Number of peaks

1493 (qval < 1E-05)

dm3

Number of total reads

13124734

Reads aligned (%)

93.0

Duplicates removed (%)

18.7

Number of peaks

0 (qval < 1E-05)