Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Larvae
Cell type
Neural stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
neural stem cells
cell type
neuroblasts
tissue
L3 larval brain
chip antibody
H3K4me3 (Millipore 07-473)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
NBs and neurons were isolated according to Harzer et. Al (2013, Nature protocols). Samples were MNase treated. Libraries were prepared according to Bowman et. Al (2013 BMC Genomics).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
39064443
Reads aligned (%)
94.5
Duplicates removed (%)
55.4
Number of peaks
7622 (qval < 1E-05)

dm3

Number of total reads
39064443
Reads aligned (%)
94.8
Duplicates removed (%)
54.1
Number of peaks
8044 (qval < 1E-05)

Base call quality data from DBCLS SRA