GSM1426323: KO H3K4me3 Chip-seq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic Stem cells
genotype
BAF250a Knockout
strain
129/C57BL/6
antibody
H3K4me3
antibody manufacturer
Millipore
antibody catalog number
07-473
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ESCs were fixed in 1% Formaldehype 10 minutes at room temperature. Nuclei were isolated and treated with micrococal nuclease followed with brief sonication. ChIP was performed on the lysate using H3K4me3 antibody. ChIP DNA was purified, end repaired, dA tailing and ligated to illumina sequencing primers. ChIP DNA was size selected and amplified then sequenced on HiSeq 2000 to generate 50 bp reads.