Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

H9

cell type

human embryonic stem cells

cell line

H9

condition

CCDC144NL-AS1 KD

passages

35

chip antibody

H3K27me3 (ACTIVE MOTIF, 39155)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq, total RNA of cells was extracted by RNAiso Plus (Takara), according to the manufactures' instructions. For ChIP-seq, lysates were clarified from sonicated nuclei and histone-DNA complexes isolated with antibody RNA libraries were prepared for sequencing using standard Illumina protocols. ChIP-seq libraries were prepared for sequencing using TruSeq Nano DNA Sample Prep Kit (Illumina), according to the manufacter's instructions.

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

21225783

Reads aligned (%)

93.1

Duplicates removed (%)

15.2

Number of peaks

3974 (qval < 1E-05)

hg19

Number of total reads

21225783

Reads aligned (%)

92.4

Duplicates removed (%)

15.7

Number of peaks

3623 (qval < 1E-05)