Curated Sample Data


Genome
dm3
Antigen Class
Input control
Antigen
Input control
Cell type Class
Cell line
Cell type
S2

Cell type information


Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by Original Data Submitter


source_name
S2 cells
antibody
input
genotype/variation
Dicer2 kd rescue Scramble
cell line
S2 cells
passages
E20-24h
chip antibody
none

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP sequencing, DNA libraries were constructed using the NEBNext® UltraTM II DNA Library Prep kit (NEB, USA) following manufacturer's recommendations. After end Repair, 5 ́ phosphorylation and dA-Tailing of purified DNA fragments, NEBNext Adaptor with hairpin loop structure were ligated and he library fragments were purified with SPRIselect sample purification beads (NEB, USA). At last, PCR products were purified, and library quality was assessed on the Agilent Bioanalyzer 2100 system. The libraries were sequenced at the Annoroad Gene Technology (Beijing, China) on an Illumina HiSeq X Ten platform and 150 bp paired-end reads were generated.

Platform Information


instrument_model
Illumina HiSeq X Ten

External Database Query

Logs in read processing pipeline


Number of total reads
3626730
Reads aligned (%)
97.7
Duplicates removed (%)
10.8
Number of peaks
1109 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA