Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BMI1

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
293T-Rex
cell line
293T-Rex
transgene
N3-SCML2A
epitope tag
Flag-HA-OneSTrEP (N-terminal)
antibody
PCGF4 - Bethyl A301-694A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared according to manufacturer’s instructions (Illumina). Immunoprecipitated DNA was first end-repaired using End-It Repair Kit (Epicenter), tailed with an A using Klenow exo minus (NEB M0212), and ligated to custom adapters with LigaFast (Promega, city, state #M8225). Fragments of 300±50 bp were size-selected and subjected to ligation-mediated PCR amplification (LM-PCR), using Phusion DNA polymerase (NEB M0530).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
26239168
Reads aligned (%)
61.3
Duplicates removed (%)
26.2
Number of peaks
1064 (qval < 1E-05)

hg38

Number of total reads
26239168
Reads aligned (%)
62.8
Duplicates removed (%)
25.0
Number of peaks
1061 (qval < 1E-05)

Base call quality data from DBCLS SRA