Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Adult

Cell type

Ovary

NA

NA

Sample

source_name

ovaries

genotype

SetDB1 mutant

developmental stage

adult

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

50 egg chambers were dissected for each sample. DNA was isolated using phenol-chloroform method. 300ng of genomic DNA was digested by DpnI endonuclease. Digested DNA was ligated with double-stranded DNA adapters and then digested with DpnII endonuclease. Next, methylated fragments were selectively amplified using adapter-specific primer. Before the preparation of libraries for Illumina DamID-seq, the adapters used in DamID procedure were cut with DpnII enzyme. No further fragmentation was performed.

Sequencing Platform

instrument_model

Illumina MiSeq

dm6

Number of total reads

1601766

Reads aligned (%)

87.4

Duplicates removed (%)

57.1

Number of peaks

39 (qval < 1E-05)

dm3

Number of total reads

1601766

Reads aligned (%)

87.6

Duplicates removed (%)

56.4

Number of peaks

38 (qval < 1E-05)