Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

neural stem cells

cell type

primary neural stem cells

primary source

SVZ

treatment of chip material

without RNase

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

2X107 NSCs were harvested, washed and cross-linked with 4% PFA for sonication. Immunoprecipitation was performed with 4 μg rabbit or goat anti-Ars2 antibody. Normal rabbit or goat IgG was used as control. For ChIP with RNase treatment, RNase A (Roche 11579681001) was added to cell pellet and/or chromatin extract to 100 μg/ml. DNA libraries were made using the Illumina ChIP-seq library preparation kit and sequenced as individual lanes on the Illumina GAII.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

184114080

Reads aligned (%)

79.7

Duplicates removed (%)

65.3

Number of peaks

7002 (qval < 1E-05)

mm9

Number of total reads

184114080

Reads aligned (%)

79.6

Duplicates removed (%)

65.3

Number of peaks

7302 (qval < 1E-05)