Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Embryo

Cell type

Hindlimb

MeSH Description

Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)

Sample

source_name

Hindlimb

strain

C57BL/6

tissue

Hindlimb

developmental stage

E10.5

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Around 500 E10.5 (36so) wild-type FLs and 500 E10.5 wild-type HLs were fixed according to standard procedures for ChIP experiments. For the IP, we used ChIP-IT High Sensitivity Columns (Active Motif). Two anti-Meis antibodies were used simultaneously, one recognizing Meis1a and Meis2a,c and the other recognizing all Meis1 isoforms. ChIP-seq libraries were generated using the NEBNext Ultra DNA Library Prep Kit for Illumina and sequenced (single-end mode and length of 50 bp) on a HiSeq 2500 (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

65735034

Reads aligned (%)

89.8

Duplicates removed (%)

40.7

Number of peaks

981 (qval < 1E-05)

mm9

Number of total reads

65735034

Reads aligned (%)

89.7

Duplicates removed (%)

40.7

Number of peaks

1029 (qval < 1E-05)