KMT2A-MLLT3 leukemia cells with low levels of DOT1L overexpressed
disease state
KMT2A-MLLT3 AML
spike-in
drosophila melanogaster (S2) cells spiked in at a 1:2 ratio
treatment
DOT1L transduction
cell population
low expressing population
chip antibody
H3K79me2 (Abcam: ab3594)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Briefly, samples were crosslinked, lysed, sonicated and immunoprecipated with H3K79me2 overnight. After a series of washes they were reverse crosslinked overnight and DNA extracted. Library prep was performed using Illumina HiSeq, 2x150bp configuration, single index, per lane. ~350M raw paired-end reads per lane