Classic GC DZ and LZ B cells were sorted using FACS. Classic GC DZ B cells were defined as B220+GL7+CD95+CXCR4+CD83lo, and classic GC LZ B cells were defined as B220+GL7+CD95+CXCR4loCD83+.
chip antibody
H3K27Ac
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from the indicated flow sorted GC B cell population (1-2 million cells) was used for each ChIP experiment with anti-H3K4me1 (Millipore 07-436) or anti-H3K27Ac (Abcam ab4729) antibody. DNA libraries were prepared by tagmentation from the sheared chromatin (200–600 bp). Libraries were sequenced on the Illumina Hiseq4000.