Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Embryo

Cell type

Forelimb

MeSH Description

A front limb of a quadruped. (The Random House College Dictionary, 1980)

Sample

source_name

E10.5 forelimb bud

strain background

mixed

tissue/chip rxn

single pair of limb buds

age

E10.5 (33-34S)

genotype

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Limb buds were dissected and dissociated prior to fixation. ChIP libraries were prepared with True microChIP library*** with 12 cycles of library amplifications. Excess primers were removed from PCR reactions using AmpureXP beads (A63880) at a ratio of 0.8x beads. DNA library- Diagenode True MicroChIP

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

59158815

Reads aligned (%)

97.7

Duplicates removed (%)

12.9

Number of peaks

679 (qval < 1E-05)

mm9

Number of total reads

59158815

Reads aligned (%)

97.5

Duplicates removed (%)

12.8

Number of peaks

765 (qval < 1E-05)