A front limb of a quadruped. (The Random House College Dictionary, 1980)
Attributes by original data submitter
Sample
source_name
E10.5 forelimb bud
strain background
mixed
tissue/chip rxn
single pair of limb buds
age
E10.5 (33-34S)
genotype
wild type
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Limb buds were dissected and dissociated prior to fixation. ChIP libraries were prepared with True microChIP library*** with 12 cycles of library amplifications. Excess primers were removed from PCR reactions using AmpureXP beads (A63880) at a ratio of 0.8x beads. DNA library- Diagenode True MicroChIP